GW-6604 is an ALK5 inhibitor with an IC 50 value of 140 nM for inhibiting its autophosphorylation, and can be used in the study of liver fibrosis. In Vitro GW-6604 (0-10 μM, ) inhibits TGF-β-induced PAI-1 transcription and secretion in HepG2 cells with an IC 50 value of 500 nM, effectively blocking the Smad-dependent response. MCE has not independently confirmed the accuracy of these methods. They are for reference only. In Vivo GW-6604(p.o., 25-80 mg/kg, twice a day, 3 weeks) dependently inhibits hepatic COL IA1 overexpression in an acute DMN model in SD rats, reducing its expression by 80%. Normal liver weight was maintained, liver fibrosis was effectively reduced and matrix degeneration was increased . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid