制备说明 This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL. General Description Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger . It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes. Chemical structure: peptidyl nucleoside Application Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger . It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL. Puromycin dihydrochloride from Streptomyces alboniger has been used: in the preparation of puromycin stock solution for puromycin sensitivity assay. to select lentivirus-infected cells containing puromycin resistant selection marker. Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C. Other Notes Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place