PACAP-38 (31-38), human, mouse, rat is a PAC 1 receptor activator and increases the α-secretase activity. PACAP-38 (31-38), human, mouse, rat elevates cytosolic Ca 2+ , increases proliferation and increases phosphorylation of extracellular regulates kinase (ERK) and the epidermal growth factor receptor (EGFR). PACAP-38 (31-38), human, mouse, rat demonstrates potent, efficacious, and sustained stimulatory effects on sympathetic neuronal NPY and catecholamine production. PACAP-38 (31-38), human, mouse, rat can be used for neurotrophic and neuroprotective research . In Vitro PACAP-38 (31-38), human, mouse, rat (100 nM; 2 min; NCI-H838 cells) induces EGFR, HER2 and ERK tyrosine phosphorylation. PACAP-38 (31-38), human, mouse, rat (100 nM; 30 min; NCI-H838 cells) induces EGFR tyrosine phosphorylation with generates ROS and increases ROS levels by 51%. PACAP-38 (31-38), human, mouse, rat (10 nM; 48 h) stimulates the growth of NCI-H838 cells. PACAP-38 (31-38), human, mouse, rat (300 nM; 4 h) stimulates generation of APPsα in neural cells. PACAP-38 (31-38), human, mouse, rat (0.01-10 nM; HEK 293 cells) stimulates neural cells express endogenous PAC1 receptors by cAMP accumulation and by an increase in cytosolic free calcium and induces elevation of the intracellular Ca 2+ concentration in a dose-dependent manner with an EC 50 value of 0.81 nM. PACAP-38 (31-38), human, mouse, rat (0.01 nM; 48 h) elicits potent and efficacious stimulation of NPY secretion from SCG neuronal cultures. PACAP-38 (31-38), human, mouse, rat (100 nM; 14 d) produces sustained stimulated NPY and catecholamine secretionmpathetic neurons. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: NCI-H838 cells Concentration: 10 nM Incubation Time: 48 hours Result: Increased the number of NCI-H838 cells by 72%. Western Blot AnalysisCell Line: 100 nM Concentration: 100 nM Incubation Time: 2 minutes Result: Increased tyrosine phosphorylation of the EGFR, HER2, and ERK by 377, 299 and 216%, respectively. Form:Solid