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Product details
名称
重组胰蛋白酶消化液(不含酚红与EDTA)
分子类型
生物试剂/缓冲液
别名
重组胰蛋白酶消化液
英文别名
Recombinant Trysin Solution
英文名称
Recombinant Trypsin Solution (W/O Phenol Red & EDTA)
货号
R1201958-100ml
包装规格
100ml
级别
BioReagent
浓度
0.025 mg/mL
储存温度
-20°C储存
运输条件
超低温冰袋运输
稳定性与储存
短期储存4℃(1-2周);长期储存-20℃(12个月);避免反复冻融。
产品介绍
胰蛋白酶(Trypsin)是胰脏分泌的一种丝氨酸肽链内切酶,识别位点为赖氨酸或者精氨酸,切割位点为精氨酸或者赖氨酸残基中的羧基端。本产品为毕赤酵母表达的重组猪胰蛋白酶,在符合GMP法规的环境下生产,不含任何动物源成分,无动物源性的病毒污染(如猪流感病毒、猪细小病毒等),天然缺乏糜蛋白酶活性。本品活性受丝氨酸蛋白酶抑制剂如PMSF和TLCK等、金属离子螯合剂如EDTA等的抑制。 本品不含EDTA,pH=7.4。 酶活定义:25℃,pH7.6,反应体系3.2ml(1cm 光路),每分钟酶解BAEE使253nm下的吸收值增加0.003定义为一个USP单位。 使用说明: 1、使用前将重组胰蛋白酶消化液和完全培养基37℃预热。 2、弃去培养液上清,用适量DPBS洗涤细胞,弃去DPBS。 3、加入适量的重组胰蛋白酶消化液(0.5ml/10cm²),37℃培养直至细胞脱落。 4、加入5-10ml的预热完全培养基,轻柔的混匀细胞,将细胞悬液转移至无菌15ml离心管中,100g离心5-10分钟。 5、弃去上清,加入适量的完全培养基进行培养。 注意事项: 1、本品收到后尽量避免反复冻融。 2、使用时请穿实验服并佩戴一次性手套。 3、本品不得直接用于临床诊断和治疗。
pH
7.4
Safety
「No Dangerous Attributes」
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Research chemical 100ml; 0. 025 mg

Research chemical 100ml; 0. 025 mg; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 100ml; 0. 025 mg.

item number:R1201958-100ml
Product model:100ml
level: 100ml; 0. 025 mg
Lead Time:30days
sold 843 Items
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100ml

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