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Product details
名称
Express 酶 (1×), 无酚红
分子类型
生物试剂/缓冲液
别名
重组胰蛋白酶类似物消化液 (1×, 不含酚红)
英文名称
Express Enzyme (1), W/O Phenol Red
货号
R1202262-100ml
包装规格
100ml
级别
无菌过滤
浓度
储存温度
2-8°C储存,避免反复冻融
运输条件
冰袋运输
稳定性与储存
长期储存2-8℃(12个月)。避免反复冻融。
产品介绍
本品为毕赤酵母表达的重组胰蛋白酶类似物,来源于微生物,识别位点为赖氨酸或者精氨酸,切割位点为精氨酸或者赖氨酸残基中的羧基端。其活性受丝氨酸蛋白酶抑制剂如PMSF和TLCK等的抑制。 本品生产过程不涉及任何动物源成分,从根源上杜绝了诸如猪流感病毒、猪细小病毒等动物源性病毒污染的风险。且其天然缺乏糜蛋白酶活性,是猪或牛源胰蛋白酶的替代品。本品消化细胞作用温和,并且性质稳定、易于存储。 使用说明: 1、使用前将Express 酶 (1×), 无酚红和完全培养基37℃预热。 2、弃去培养液上清,用适量DPBS洗涤细胞,弃去DPBS。 3、加入适量的Express 酶 (1×), 无酚红(0.5ml/10cm²),37℃培养直至细胞脱落。 4、加入5-10mL的预热完全培养基,轻柔的混匀细胞,将细胞悬液转移至无菌15mL离心管中,100g离心5-10分钟。 5、弃去上清,加入适量的完全培养基进行培养。 优势: 1、重组表达,无动物源成分。 2、无其他蛋白酶污染,如糜蛋白酶。 3、对细胞毒性小,消化更温和,更适合敏感细胞。 4、来源于微生物,稳定性更高,可在2-8℃长期保存。 注意事项: 1、避免反复冻融。 2、使用时请穿实验服并佩戴一次性手套。 3、本品不得直接用于临床诊断和治疗。
pH
7.2-7.5
Safety
「No Dangerous Attributes」
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「No upstream and downstream information yet」
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Research chemical 100ml

Research chemical 100ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 100ml.

item number:R1202262-100ml
Product model:100ml
level: 100ml
Lead Time:30days
sold 10 Items
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Specifications:

100ml

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