核糖核酸酶T1 来源于米曲霉(色谱纯化)

核糖核苷-3'-脒基-寡核苷酸水解酶

R128610-100KU

100KU

EnzymoPure™

≥300,000 units/mg protein

Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA. Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies . Application Ribonuclease T1 has extensive applications in molecular cloning and DNA sequencing. Because of its specificity it has been a commonly used cleavage enzyme for the determination of structure, nearest neighbor frequencies, and RNA sequencing. The enzyme has further application in the preparation of nucleoside 2',3'-cyclic phosphates, the synthesis of oligonucleotides, and the removal of RNA from DNA preparations. The enzyme is also used as a non-mammalian source of RNase in various applications.

来自黑曲霉（Aspergillus oryzae）的核糖核酸酶 T1（RNase T1）是一种内切核糖核酸酶，可水解 G 残基。裂解发生在胍核糖核苷酸的 3'- 磷酸基团和相邻核苷酸的 5'- 羟基之间。最初的产物是 2'：3'环磷酸核苷，然后水解为相应的 3'-核苷磷酸。它与胰腺 RNase 的不同之处在于，它专门攻击鸟嘌呤位点，生成 3′-GMP 和带有 3′-GMP 末端基团的寡核苷酸。