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名称

RPMI 1640 培养基, 不含谷氨酰胺

分子类型

培养基

别名

RPMI 1640 培养基，不含谷氨酰胺

英文别名

RPMI 1640 Medium, no glutamine

英文名称

RPMI 1640 Medium, no L-Glutamine

货号

R1372073-500ml

包装规格

500ml

级别

无菌过滤

储存温度

2-8°C储存,避光

运输条件

冰袋运输

稳定性与储存

Store at 2-8℃ long term (12 months). Store in the dark.

敏感性

Light-sensitive

产品介绍

RPMI 1640培养基以研发地点罗斯韦尔公园纪念研究所（Roswell Park Memorial Institute. RPMI）命名，1640为培养基代号。它是McCoy's 5A培养基的改进型，使用碳酸氢盐缓冲系统。RPMI1640培养基最初开发用于人白血病细胞的悬浮或单层培养，后来被发现也适用于多种哺乳动物细胞，包括HeLa、Jurkat、MCF-7、PC-12、PBMC、星形胶质细胞和癌细胞，尤其适用于悬浮细胞的培养，是使用最为广泛的培养基之一。 RPMI 1640培养基与其它培养基的区别在于含有还原型谷胱甘肽和高浓度的维生素。 RPMI 1640培养基含有EMEM和DMEM中没有的生物素、维生素B12和对氨基苯甲酸，以及高浓度的氯化胆碱和肌醇。L-谷氨酰胺是细胞培养液体环境中所必需的一种营养成分，但其在水溶液中不稳定，易降解。不含L-谷氨酰胺的培养基，可以根据研究需求，自行调整L-谷氨酰胺的含量，在使用前添加新鲜的L-谷氨酰胺或其替代物更利于细胞的生长。本产品含有：D-葡萄糖、酚红。不含有：L-谷氨酰胺、丙酮酸钠、HEPES。

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

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