ROS Probe，HPF是一种细胞可渗透的，光稳定的羟苯基取代的荧光素化合物，可作为检测羟基自由基和过氧亚硝酸盐的高度灵敏的荧光探针。该化合物对其他活性氧（ROS），例如次氯酸盐，单线态氧，超氧化物，过氧化氢，一氧化氮和烷基过氧化物几乎没有活性。 ROS Probe, HPF is a cell-permeable, photo-stable hydroxyphenyl-substituted fluorescein compound that acts as a highly sensitive fluorescent probe for the detection of hydroxyl radical and peroxynitrite. The compound has little activity towards other reactive oxygen species (ROS), such as hypochlorite, singlet oxygen, superoxide, hydrogen peroxide, nitric oxide and alkyl peroxide. Purpose Hydroxyphenyl fluorescein (HPF) is the reagent that can directly detect highly reactive oxygen species (hROS). Hydroxyphenyl fluorescein selectively and dose-dependently reacts with hROS, such as the hydroxyl radical and peroxynitrite, which exhibit strong fluorescence