红色荧光团标准。广泛用于酶测定系统。试卤灵的吸收和荧光是 pH 依赖性的。低于 pKa（约 6.0），吸光度转移到约 480 nm、E 和荧光量子产率都明显降低。在二硫苏糖醇（DTT）和 2-巯基乙醇等硫醇存在下，试卤灵是不稳定的。 试卤灵具有高消光系数和良好的化学和光稳定性。试卤灵的这些性质使其非常适合用于反向荧光法检测H2O2，通过测定辣根过氧化物酶介导其氧化成非荧光衍生物时荧光的消失来检测 。 Red fluorescent group standard. It is widely used in enzyme determination system. The absorption and fluorescence of trihalogenase are pH dependent. The absorption transfer to about 480nm, e and fluorescence quantum yield decreased significantly. In the presence of thiols such as DTT and 2-mercaptoethanol, the experimental haloling is unstable. The experimental halogenase has high extinction coefficient and good chemical and light stability. These properties of trihaloling make it very suitable for the detection of H2O2 by reverse fluorescence method. The fluorescence disappearance of horseradish peroxidase mediated oxidation into non fluorescent derivatives is determined.