RNA Extraction Buffer (氯仿替代品)

生物试剂/缓冲液

氯仿替代品 | 氯仿替代物 | RNA提取辅助试剂(氯仿替代物) | RNA提取辅助试剂

RNA Extraction Agent

RNA Extraction Buffer

R749970-50ml

50ml

BioReagent

避光,室温

常规运输

Store at Room temperature long term (24 months). Upon receipt, it is recommended to aliquot. Store in the dark.

Light sensitive

产品介绍： 氯仿替代物主要用于在Trizol等RNA抽提试剂相关的RNA抽提过程中可以替代氯仿的试剂。本产品毒性低，可以代替氯仿使用。使用方法与氯仿相同;不会对分离的RNA的质量或数里产生不利影响。 注意事项： 1.如果每次的使用里很小，可以适当分装后再使用，以免污染。 2.为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明： 1. 样本预处理后，加1mL TRIzol试剂，裂解样本，室温孵育5min。 2. 加0.1mL 氯仿替代物，振荡混匀，孵育3min。 3. 在4C室温下12000xg离心样本15min。 4. 取上层水样层约500uL至新样本管中。离心后混合物分成三层:下层有机层，中间层，上层无色的水样层。RNA存在于上层水样层当中。 5. 加等样本体积的异丙醇，颠倒混匀，室温下孵育10min。 6. 在4C室温下12000xg离心10min，弃上清。 7. 加1mL 75%乙醇洗涤沉淀，在4C室温下12000xg离心3-5min，弃上清液。此步骤重复一次。 注意：剩余的少里液体可短暂离心，然后用枪头吸出，注意不要吸弃沉淀。 8. 室温干燥3-5min，加20-50μL RNase-free H2O，充分溶解沉淀。(可在55°C水浴加速溶解) 注意：沉淀不要过分干燥，以免难于溶解。 Product Introduction: Chloroform substitutes are mainly used to replace chloroform reagents in RNA extraction processes related to Trizol and other RNA extraction reagents. This product has low toxicity and can be used as a substitute for chloroform. The usage method is the same as chloroform; It will not have any adverse effects on the quality or quantity of isolated RNA. Precautions ： 1. If the usage is small each time, it can be packaged appropriately before use to avoid contamination. 2. For your safety and health, please wear lab clothes and disposable gloves when operating. Instructions for Use： 1. After sample pretreatment, add 1mL of TRIzol reagent, lyse the sample, and incubate at room temperature for 5 minutes. 2. Add 0.1mL chloroform substitute, shake well, and incubate for 3 minutes. 3. Centrifuge the sample at 12000xg for 15 minutes at room temperature of 4C. 4. Take about 500uL of the upper water sample layer and transfer it to a new sample tube. After centrifugation, the mixture is divided into three layers: the lower organic layer, the middle layer, and the upper colorless water sample layer. RNA exists in the upper aqueous layer. 5. Add isopropanol of equal sample volume, invert and mix well, and incubate at room temperature for 10 minutes. 6. Centrifuge at 12000xg for 10 minutes at room temperature 4C and discard the supernatant. 7. Wash the precipitate with 1mL of 75% ethanol, centrifuge at 12000xg at room temperature for 3-5 minutes, and discard the supernatant. Repeat this step once. Attention: The remaining small liquid can be briefly centrifuged and then sucked out with a gun tip, being careful not to discard the sediment. 8. Dry at room temperature for 3-5 minutes, add 20-50 μ L RNase free H2O, and dissolve the precipitate thoroughly. (Can dissolve rapidly in a 55 ° C water bath.) Attention: Do not dry the sediment excessively to avoid difficulty in dissolution.