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名称
Gelred-prestained DNA Ladder (100-5000bp)
别名
Gelred预染DNA分子量标准
英文别名
Gelred-prestained 250bp-II DNA ladder | Gelred-prestained DNA Marker
英文名称
Gelred-prestained DNA Ladder (100-5000bp)
货号
R751622-500T
包装规格
500T
级别
适用于电泳
浓度
100-5000bp, 9 bands, 100bp/ 250bp/ 500bp/ 750bp/ 1000bp/ 1500bp/ 2000bp/ 3000bp/ 5000bp
储存温度
-20°C储存,避免反复冻融
运输条件
超低温冰袋运输
稳定性与储存
Store at room temperature short term (1 months). Store at 4℃ long term (18 months). Store at -20℃ long term (36-60 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
产品介绍
储存缓冲液:GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6 产品说明 1、本产品是由9条线状双链DNA片段组成,大小范围为100bp~5000bp,分别为100bp,250bp,500bp,750bp,1000bp,1500bp,2000bp,3000bp,5000bp。500bp和1500bp为加亮带指示,浓度为其它条带的2.5倍,便于电泳后观察。 2、5ul本产品中,常规条带的含量约为30ng,加亮带含量约75ng。 3、本产品已保存在 1xLoading Buffer 中,可直接用于电泳,使用方便。 4、本产品及配套的5xLoading buffer均已包含了Gelred核酸染料,配套使用,电泳后,可直接在紫外下观察,无需做后续染色处理。 5、本产品不适合于聚丙烯酰胺凝胶电泳。 6、推荐电泳缓冲液及琼脂糖凝胶浓度: 1xTAE电泳缓冲液 琼脂糖浓度:1.5%~2.0% 7、使用本产品体系,琼脂糖凝胶中无需加任何核酸染料。 使用说明 1、制备合适浓度的,不含任何核酸染料的琼脂糖凝胶。 2、凝胶浓度对DNA电泳的影响比较大,本品建议的琼脂糖凝胶浓度为1.5%~2.0%。 3、建议使用1xTAE缓冲液,电泳电压不超过10v/cm。 4、一般常见的3.5mm加样孔,DNA marker建议用量3~5ul,宽胶孔需适当增加样品量。 5、将待检测的样品与配套的5xLoading buffer,按照约4:1的比例混合,然后加入凝胶加样孔。 6、电泳至合适距离: 由于Gelred与DNA结合牢固,可以充分利用凝胶长度,电泳更长的距离,只要最小片段不要跑出凝胶即可,以有利于小片段电泳分离。一般溴酚蓝指示带距凝胶边缘不小于1cm。 7、电泳结束后,在紫外灯下观察电泳条带。 8、产品内所附的5x Loading buffer 用于与待检测样品混合后点样使用,含溴酚蓝和二甲苯青双指示剂。 9、如果是有大量可以直接点样的样品需要电泳检测,建议用Gelred制胶法来检测,不用预混样品,可以大量节约实验时间。 组分表 R751622
pH
8.0-8.5
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Research chemical

Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:R751622-500T
Product model:500T
level: Suitable for electrophoresis
Lead Time:30days
sold 311 Items
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