RO-5963 is a dual p53-MDM2 and p53-MDMX inhibitor with IC 50 s of ~17 nM and ~24 nM, respectively In Vitro RO-5963 (10-20 μM; 48 hours) shows much higher apoptotic activity than Nutlin in both MCF7 and ZR75-30 cell lines. RO-5963 (10 μM, 24 hours) effectively activats p53 and elevates p21 and MDM2 levels. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Apoptosis AnalysisCell Line: ZR75-30, MCF7 cells Concentration: 10, 20 μM Incubation Time: 48 hours Result: Potently showed apoptotic activity. Western Blot AnalysisCell Line: LNCap, U2OS, RKO, A489, 22Rv1, HCT116, H460, LOX, MCF7, A549, G401 cells Concentration: 10 μM Incubation Time: 24 hour Result: Effectively activated p53 and elevated p21 and MDM2 levels. Form:Solid IC50& Target:IC50: ~17 nM (p53-MDM2), ~24 nM (p53-MDMX)