羧肽酶B(质谱级)

序列分析纯重组羧肽酶B | 肽酰 - L - 赖氨酸 (L - 精氨酸) 水解酶 | 精蛋白酶

DTXSID801009277 | FT-0623486 | Carboxypeptidase B from human pancreas, 50-55 units/mg protein carboxypeptidase B | 4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobu

S141076-100μg

100μg

无动物源

≥95%(HPLC)

细胞定位：Cytoplasmic vesicle. Secreted. 序列相似性 ：Belongs to the peptidase M14 family. 羧肽酶 B (Carboxypeptidase B) 又称肽酰 - L - 赖氨酸 (L - 精氨酸) 水解酶、精蛋白酶，是一种金属蛋白酶，可专一水解蛋白质 C - 末端碱性氨基酸 (赖氨酸、精氨酸、组氨酸)。 酶切位点：羧肽酶B专一水解蛋白质C-末端碱性氨基酸（赖氨酸(Lysine、K)、精氨酸(Arginine、R)、组氨酸(Histidine、H)）的氨基端； 氨基酸序列 ：雅心重组羧肽酶B是由重组大肠杆菌表达生产，氨基酸序列与大鼠胰腺羧肽酶B完全一致。 酶学性质 ：具有与动物源性羧肽酶B相同的酶学性质，可替代动物源性羧肽酶B用于各种生物技术过程中。 基本特性 ：又称为肽酰-L-赖氨酸（L-精氨酸）水解酶；精蛋白酶。分子量为33.8kD，等电点为6.0，最适pH为7.5-9.0。活性受精氨酸、赖氨酸的竞争性抑制，金属离子螯合剂如EDTA等抑制酶活。 使用方法 溶解 ：终浓度1-10mg/ml溶解在纯水或25mM Tris-HCl（pH 7.5-8.0）中。 使用 ：酶量：目的蛋白=1:20-1:100（w:w），最适酶切pH为7.0-9.0。 储存稳定性 重组羧肽酶B冻干粉存于2-8℃，24个月稳定；用无菌水或者25mM Tris-HCl （pH 7.6）溶解后存于-20℃，反复冻融10次，无活性损失。 产品优势 序列分析纯蛋白酶 ：特异性高，稳定性好。 无动物源性 ：重组生产，无外源性的病毒污染，生产过程不使用任何动物源原料。 质量稳定 ：批量生产，可保证稳定连续的批次生产；产品批次间无差异，质量稳定。 纯度高 ：比活高；宿主蛋白残留小于生物制品限度要求。 冻干粉 ：易于储存和运输。 符合法规要求 ：生产设备和生产环境符合相关法规要求，生产过程完全遵循NSF ISO 9001:2015质量体系并符合GMP指导原则。 质量文件完整 ：按客户需求，可提供相关法规支持文件。 产品用途 抗体碱性峰的检测; 蛋白质C-末端氨基酸的测定。 Carboxypeptidase B (Carboxypeptidase B), also known as peptidyl - L - lysine (L - arginine) hydrolase and arginase, is a metalloprotease that can specifically hydrolyze basic amino acids (lysine, arginine, histidine) at the C - terminus of proteins. Enzymatic cleavage site : Carboxypeptidase B specifically hydrolyzes the amino terminus of basic amino acids (lysine (Lysine, K), arginine (Arginine, R), histidine (Histidine, H)) at the C - terminus of proteins. Amino acid sequence : Yaxin recombinant carboxypeptidase B is produced by recombinant Escherichia coli expression, and its amino acid sequence is completely consistent with that of rat pancreatic carboxypeptidase B. Enzymological properties : It has the same enzymological properties as animal - derived carboxypeptidase B and can replace animal - derived carboxypeptidase B in various biotechnology processes. Basic characteristics : Also known as peptidyl - L - lysine (L - arginine) hydrolase; arginase. The molecular weight is 33.8 kD, the isoelectric point is 6.0, and the optimal pH is 7.5 - 9.0. Its activity is competitively inhibited by arginine and lysine, and metal ion chelators such as EDTA inhibit the enzyme activity. Usage Method Dissolution : Dissolve to a final concentration of 1 - 10 mg/ml in pure water or 25 mM Tris - HCl (pH 7.5 - 8.0). Usage : Enzyme amount: target protein = 1:20 - 1:100 (w:w), and the optimal enzymatic cleavage pH is 7.0 - 9.0. Storage Stability The lyophilized powder of recombinant carboxypeptidase B is stable for 24 months when stored at 2 - 8°C; after being dissolved in sterile water or 25 mM Tris - HCl (pH 7.6) and stored at - 20°C, there is no loss of activity after repeated freezing and thawing for 10 times. Product Advantages Sequence - analyzed pure protease: high specificity and good stability. Animal - free origin : produced by recombination, free from exogenous virus contamination, and no animal - derived raw materials are used in the production process. Stable quality : mass production can ensure stable and continuous batch production; there is no difference between product batches, and the quality is stable. High purity : high specific activity; residual host protein is lower than the limit requirements for biological products. Lyophilized powder : easy to store and transport. Compliance with regulatory requirements : production equipment and production environment meet relevant regulatory requirements, and the production process fully follows the NSF ISO 9001 :2015 quality system and conforms to GMP guidelines. Complete quality documents : relevant regulatory support documents can be provided according to customer needs. Product Applications Detection of antibody basic peaks; Determination of amino acids at the C - terminus of proteins.

Preferential release of a C-terminal lysine or arginine amino acid.

≥200 U/mg protein

重组表达

33.6 kDa

E.coli & Pichia pastoris Accession #: P19223 | E.coli & Pichia pastoris

大鼠(Rat)

Yes

Yes