琥珀酰亚胺戊酸酯 PEG, mPEG-SVA

Succinimidyl valeric acid PEG, mPEG-SVA

S164337-1g

1g

MW 30000 Da

-20°C储存

超低温冰袋运输

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol valeric acid (PEG-SVA) is an amino (-NH 2 ) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl valeric acid PEG (mPEG-SVA) offers superior reactivity yet higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH. N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol valeric acid (PEG-SVA) is an amino (-NH 2 ) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl valeric acid PEG (mPEG-SVA) offers superior reactivity yet higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

一般来说，NHS PEG 摩尔过量 10 到 50 倍于含胺材料的量就能充分缀合：PEG NHS 储存液：50 mg 加入 1 mL 结合缓冲液：反应步骤：将目标材料溶解在连接缓冲液中。估计目标材料上伯胺基团的浓度。向目标连接材料中加入 NHS PEG 原液，最终浓度至少保持在 10 mg/mL。最佳连接需要 10~50 摩尔过量的 PEG NHS；让混合物在室温下搅拌 30~60 分钟或在 4 0C 下搅拌 2 小时。注：此处显示的结构主要代表来自 PEG 的官能团，可能无法显示确切的连接体。如果您对连接体有任何疑问，请联系我们。

Off-white/white solid or viscous liquid depends on molecule weight;10 mg/mL, clear in water, DMSO, Chloroform;