使用四甲基罗丹明-5-马来酰亚胺： 利用外源荧光探针在实验用Cys48上衍生绿色荧光蛋白(GFP)； 培养单溶剂显露的半胱氨酸突变以进行蛋白质标记。 5-TAMRA maleimide is an orange-fluorescent, single isomer, thiol-reactive dye for synthesis of orange fluorescent biomolecules. The maleimide group is selective for reaction with thiol groups on peptides, proteins, and other thiol-containing biomolecules when reacted at pH below 7.5. 5-TAMRA maleimide, sometimes also referred to as TMRM, has been used for investigations of conformational changes in voltage sensitive domains of ion channels by site directed fluorescent labeling. Conjugates of 5-TAMRA maleimide are excited near 541 nm with fluorescence emission near 567 nm and are relatively photostable and insensitive to changes in pH. 5-TAMRA maleimide is a purified single isomer label that is suitable for preparing conjugates that are purified by capillary electrophoresis or ion-exchange chromatography. Please see the bottom of the page for other related products. Thiol-Reactive Fluorescent Probe