使用4-硝基苯基磷酸二钠盐六水合物：作为底物来测定10号染色体上缺失的磷酸酶和张力蛋白同源物(PTEN)磷酸酶活性的动力学参数作为测定酸性磷酸酶活性的底物作为底物测定抗兔/人碱性磷酸酶结合性抗体应用： 用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应，并在 405nm 下读数。 Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm. Application: 4-nitrophenyl phosphate disodium salt hexahydrate has been used: as a substrate to measure the kinetic parameters of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphatase activity as substrate to assay acid phosphatase activity as substrate to assay bound antibodies with anti-rabbit/human alkaline phosphatase conjugate