由于高度纯化的若丹明110（R110）衍生的底物被锁定为内酯构型，因此它们是无色且无荧光的。半胱天冬酶对R110肽的切割产生强荧光的R110，可以在510-530 nm处用488 nm的激发光进行荧光监测，这是荧光仪器中最常用的激发光源。R110衍生的caspase底物可能是最敏感的指示剂，广泛用于荧光检测各种caspase活性。该R110底物专门针对半胱天冬酶3和7。我们基于R110的底物经过高度纯化，以消除痕量的游离R110，这是HPLC无法检测到的，但会引起大量测定背景。 Since highly purified rhodamine 110 (R110)-derived substrates are locked in the lactone configuration they are colorless and non-fluorescent. Cleavage of R110 peptides by caspases generates strongly fluorescent R110 that can be monitored fluorimetrically at 510-530 nm with excitation of 488 nm, the most common excitation light source used in fluorescence instruments. R110-derived caspase substrates are probably the most sensitive indicators widely used for the fluorimetric detection of various caspase activities. This R110 substrate is specific for caspases 3 and 7. Our R110-based substrates are highly purified to eliminate the trace amount of free R110 that is not detectable by HPLC, but causes significant assay background. (Z-DEVD)2-R110is strongly fluorescent R110 that can be monitored fluorimetrically at 510-530 nm with excitation of 488 nm