SIRT1-IN-2 (compound 3h) is a potent and selective SIRT1 (silent information regulator 1) inhibitor, with an IC 50 of 1.6 μM In Vitro SIRT1-IN-2 (compound 3h) (0-100 μM, 48 h) inhibits the proliferation of Human cancer cell lines including K562, HCT-116, HepG2, A549, and MCF-7, and shows significantly less cytotoxic on 293T cells and HUVEC. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cytotoxicity AssayCell Line: Human cancer cell lines (K562, HCT-116, H460, HepG2, A549, HT-29, MCF-7) and normal cell lines (293T, HUVEC).Concentration: 0, 0.01, 0.1, 1, 10, 100 μM Incubation Time: 48 h Result: Inhibited the proliferation of Human cancer cell lines including K562, HCT-116, HepG2, A549, and MCF-7, with IC 50 values of 51, 37, 40, 48, and 48 μM, respectively. And showed significantly less cytotoxic on 293T cells and HUVEC, with IC 50 values of > 100 and 45 μM, respectively. Form:Solid IC50& Target:SIRT1 1.6 μM (IC 50 ) SIRT2 39 μM (IC 50 )