Salviolone is a natural diterpenoid derivative that can against melanoma cells. Salviolone exhibits a pleiotropic effect against melanoma by hampering cell cycle progression, STAT3 signaling, and malignant phenotype of A375 melanoma cells. In Vitro Salviolone (5-60 μM; 72 hours) reduces cell viability in the A375 and MeWo melanoma cell lines with EC 50 values of 17 µM and 22 µM, respectively. Salviolone does not affect the growth of normal melanocytes. Salviolone (20 μM; 48-72 hours) strongly reduces pRb, pCdk2, and cyclin A2, Tyr705-STAT3 phosphorylation expression levels in A375 cells. Salviolone also strongly increases the P21 and P53 protein expression level. Salviolone induces sustained activation of the phosphorylation of ERK1/2 and Akt. Salviolone (10-20 μM) inhibits MMP2 gelatinase activity in the A375 melanoma cell line. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: A375, MeWo melanoma cells, and NHEM cells Concentration: 5 μM, 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM Incubation Time: 72 hours Result: Impaired the viability of melanoma cells without affecting the growth of normal melanocytes. Western Blot AnalysisCell Line: A375 cells Concentration: 20 μM Incubation Time: 48 hours, 72 hours Result: Reduced the expression of the active forms of Cdk2 (pCdk2) and cyclin A2, and the phosphorylation of Rb. Form:Solid