微量分析亚硝酸的试剂。氧化还原指示剂、酸碱指示剂、生物染色剂。 高纯级用在Flemming triple染色中的染色体染色；在吉姆萨染色中对革兰氏阴性细菌染色；在Safranine-fast green FCF染色中对植物细胞染色。 Safranin T is a biological stain commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. Safranin T has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure Safranin T is used to stain for chromosomes. In late Palaeozoic pteridosperm cuticles Safranin T has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. Safranin T has also been used to detect mucin and cartilage. A biological stain used to stain Gram negative bacteria, chormosomes, mucin, cartilage and mast cells.