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Product details
名称
SDS-PAGE蛋白上样缓冲液 (2X)
分子类型
生物试剂/缓冲液
别名
双色SDS-PAGE蛋白上样缓冲液(2X) | 双色SDS-PAGE蛋白上样缓冲液(2X | 不含DTT )
英文别名
Dual Color SDS-PAGE Protein Sample Loading Buffer, 2X, Odorless | Dual Color SDS-PAGE Protein Sample Loading Buffer (2X)
英文名称
SDS-PAGE Protein Sample Loading Buffer (2X)
货号
S750310-5ml
包装规格
5ml
级别
BioReagent
浓度
2X, 无气味,不含 DTT
储存温度
-20°C储存,避免反复冻融
运输条件
超低温冰袋运输
稳定性与储存
Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
产品介绍
SDS-PAGE蛋白上样缓冲液 (2X) 是阿拉丁自主研发的一款更加安全无气味、以溴酚蓝为染料、2倍浓缩的改良型蛋白上样缓冲液。本产品从-20℃取出后可快速融化,不粘稠且节省时间;使用无味、更稳定的新型还原剂代替了DTT和β-巯基乙醇。适用于SDS-PAGE蛋白样品的上样操作。 使用说明: 1. 在室温(10~30℃)融化完全,并混匀。 注:如需要更快速使用,可置于37℃水浴或金属浴中融化,但尽量减少融化后水浴或金属浴放置时间。 2. 每20μl蛋白样品加入10μl的上样缓冲液(2倍稀释),混匀。 注:如果蛋白样品浓度过高,可以用双蒸水稀释后再加入上样缓冲液。 3. 将上述混合物放入100℃水浴加热5-10分钟,使蛋白变性。 4. 冷却至室温后,10000-14000rpm离心2-5分钟,取上清直接上样即可。 5. 通常电泳至溴酚蓝到达胶的底端附近1cm处即可停止电泳。 注意事项: 1. 无气味款和含DTT款SDS-PAGE蛋白上样缓冲液因为含有的还原剂不同,个别蛋白的电泳结果可能会有差异。 2. 本产品含有变性剂,不适用于Native-PAGE。 3. 本产品必须完全融化混匀后再使用。 4. 为了您的安全和健康,请穿实验服和佩戴一次性手套。
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Research chemical 5ml

Research chemical 5ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 5ml.

item number:S750310-5ml
Product model:5ml
level: 5ml
Lead Time:30days
sold 799 Items
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