SDS-PAGE蛋白上样缓冲液 （非还原，5X）

生物试剂/缓冲液

变性非还原蛋白上样缓冲液 | 变性非还原PAGE蛋白上样缓冲液( 5x)

enaturated PAGE protein loading buffer( Non reducing, 5X) | Denaturated PAGE protein loading buffer | Non-denatured Gel Sample Loading Buffer

SDS-PAGE Sample Loading Buffer (Non-reduced, 5X)

S767014-5ml

5ml

BioReagent

避光,-20°C储存,避免反复冻融

超低温冰袋运输

Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark.

Light sensitive

SDS-PAGE蛋白上样缓冲液 （非还原，5X）是以溴酚蓝为染料，5倍浓缩的SDS-PAGE凝胶电泳上样缓冲液，具有沉淀蛋白样品进入凝胶孔和指示蛋白样品在凝胶中运动位置的作用同时缓冲液中不含有DTT/B-巯基乙醇等还原剂，不影响可使蛋白质分子的链内二硫键和链间二硫键。但是溶液中含有SDS类变性剂。适用于变性非还原型SDS-PAGE的蛋白样品制备和上样。 注意事项: 1. 加样前在室温或 37-40℃ 数分钟解冻后轻轻摇匀，以确保溶液混合均匀; 2. 本产品仅用于科研，不能用于人体实验或人体治疗; 3. 为了您的自身安全，使用试剂前，请做好防护，如穿实验服，带手套等。 使用说明： 1、试剂准备 1.1将蛋白示踪上样缓冲液(非还原，5x)与蛋白样品按照 1:4的比例混匀; 2、蛋白样品处理 2.1 将蛋白样品置于沸水浴中加热 3-5 分钟; 2.2 待蛋白样品充分变性后冷却至室温，以小于3000rpm 的条件离心 30 秒: 3、蛋白上样及电泳 3.1离心后，以微量进样器取适量上清，直接加入 SDS-PAGE 凝胶加样孔内; 3.2进行常规电泳，通常染料到达距离凝胶底端0.5cm-1cm 处即可停止电泳。 SDS-PAGE Sample Loading Buffer (Non-reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis sample loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into the gel pores and indicating the movement position of protein samples in the gel. At the same time, the buffer does not contain reducing agents such as DTT/B-mercaptoethanol, which does not affect the disulfide bond in the chain and the disulfide bond between the chains of protein molecules. But the solution contains SDS type denaturing agents. Suitable for protein sample preparation and loading in denatured non reduced SDS-PAGE. Precautions ： 1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution; 2. This product is only used for scientific research and cannot be used for human experiments or treatments; 3. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents. Instructions for Use： 1. Reagent preparation 1.1 Mix the protein tracer loading buffer (non reducing, 5x) with the protein sample in a ratio of 1:4; 2. Protein sample processing 2.1 Heat the protein sample in a boiling water bath for 3-5 minutes; 2.2 After sufficient denaturation of the protein sample, cool it to room temperature and centrifuge at less than 3000rpm for 30 seconds: 3. Protein loading and electrophoresis 3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole; 3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.

6.2-7.2