USP8-IN-3 (Compd U51) is a deubiquitinase USP8 inhibitor with an IC 50 value of 4.0 μM. USP8-IN-3 also inhibits the proliferation of GH3 and H1957 cells with GI 50 s of 37.03 μM and 6.01 μM, respectively In Vitro USP8-IN-3 (6.25-100 μM; 15 min) inhibits the cleavage activity of USP8 on diubiquitin. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: / Concentration: 6.25 μM, 12.5 μM, 25 μM, 50 μM, and 100 μM Incubation Time: 15 min; added Di-Ub substrate for another 1 h Result: Decreased the cleaved diubiquitin level. Form:Solid IC50& Target:USP8 4.0 μM (IC 50 )