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名称

T4 多聚核苷酸激酶 (3'磷酸酶活性缺失)

英文别名

T4 PNK (3' phosphatase minus)

货号

T1372520-4×500U

包装规格

4×500U

级别

无动物源

浓度

10 U/μL

产品介绍

本公司生产的 T4 多聚核苷酸激酶(3'磷酸酶活性缺失)，即 T4 Polynucleotide Kinase (3' phosphatase minus)，简称 T4 PNK (3' phosphatase minus)是一种多聚核苷酸 5'羟基激酶，可以催化 ATP 的γ位磷酸基团向单链或双链 DNA、RNA、寡核苷酸或带有 3'磷酸基团的单核苷酸的 5'羟基转移。其它 NTP 也可产生相同的反应：5'-OH + NTP → 5'-P + NDP。与 T4 Polynucleotide Kinase 相比较，T4 Polynucleotide Kinase (3' phosphataseminus)缺失了 3'磷酸酶活性，即不能催化 3'端磷酸化多聚核苷酸的去磷酸化：3'-P → 3'-OH + Pi (最适 pH 为 5.9 左右)。T4 PNK (3' phosphataseminus)特别适用于把 OH-DNA/RNA-p 催化转变为 p-DNA/RNA-p，或把 Np 催化转变为 pNp。来源（Source）

来源

重组表达

表达系统

E. coli Accession #: NP_049834.1 | E. coli

种属

Escherichia phage T4

无动物源

Yes

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

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「No related articles yet」

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