F-ara-EdU（2'-脱氧-2'-氟-5-乙炔基尿苷）是胸苷的合成类似物，用于研究从头DNA合成和细胞增殖。它是BrdU（5-溴-2'-脱氧尿苷）和EdU（5-乙炔基-2'-去氧尿苷）的细胞毒性较小的替代品。 F-ara-EdU代替天然胸苷在细胞周期的S期结合到复制DNA中。F-ara-EdU在DNA中的代谢掺入可以通过铜催化的与荧光或生物素标记的叠氮化物的点击反应来检测。 与EdU相反，F-ara-EdU很少或根本不会引起细胞停滞或DNA合成抑制。因此，F-ara-EdU非常适合用于脉冲追踪实验，该实验旨在确定体内DNA的出生日期和长期细胞存活率。 应用： 一种比 EdU 和 BrdU 毒性都低的体内 DNA 合成探针。当需要延长细胞存活和未受干扰的细胞周期进展时，非常适合用于代谢 DNA 标记。 A probe for DNA synthesis in vivo that is less toxic than both EdU and BrdU. Ideally suited for metabolic DNA labeling when prolonged cell survival and undisturbed cell cycle progression are required. Application： F-ara-EdU (2’-deoxy-2’-fluoro-5-ethynyluridine) is a synthetic analog of thymidine used to study de novo DNA synthesis and cell proliferation. It is a less cytotoxic alternative for BrdU (5-Bromo-2’-deoxyuridine) and EdU (5-Ethynyl-2’-deoxyuridine). F-ara-EdU incorporates into replicating DNA during the S-phase of the cell cycle instead of natural thymidine. Metabolic incorporation of F-ara-EdU into DNA can be detected by copper-catalyzed click reaction with fluorescent or biotin-labeled azides. In contrast to EdU, F-ara-EdU causes little or no cellular arrest or DNA synthesis inhibition. Therefore, F-ara-EdU is ideally suited for pulse-chase experiments aimed at birth-dating DNA in vivo and long-term cell survival estimation.