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名称
TBSTx(TBS with Triton X-100)
别名
TBSTx(含 Triton X-100 的 TBS)
货号
T743333-100ml
包装规格
100ml
浓度
10X
产品介绍
阿拉丁生产的TBSTx (10X),即(TBS with Triton X-100, 10X),可以用于免疫印迹(Immunoblot, 即Western blot或WB)、免疫荧光(Immunofluorescence, IF)、免疫组化(Immunohistochemistry, IHC)和免疫细胞化学(Immunocytochemistry, IC)等实验中封闭液的配制、一抗或二抗的配制、一抗或二抗孵育后的洗涤等。封闭和洗涤可以降低背景,增强信噪比。本产品为10X浓缩液,使用时须用蒸馏水或去离子水溶解配制成1X后使用。例如配制1L TBSTx (1X),取100ml TBSTx (10X)加入900ml去离子水混匀即可。TBSTx (1X)溶液中含有20 mM Tris, 137 mM NaCl, 0.1%的Triton X-100,pH7.6±0.1 (25℃)。对于Western blot实验中膜的洗涤,按照每张膜洗涤需要5-10毫升TBSTx (1X)计算,每100ml TBSTx (10X)可以洗涤100-200次;对于免疫染色等实验,按照每个样品洗涤需要0.2-1毫升TBSTx (1X)计算,每100ml TBSTx (10X)可以洗涤1000-5000个样品。4℃保存,一年有效。-20℃可以长期保存,室温保存一个月内有效。本TBSTx (10X)配制成TBSTx (1X)后,宜4℃保存,并在一个月内用完。如果室温保存,宜在一周内用完。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。为了您的安全和健康,请穿实验服并戴一次性手套操作。 使用说明 : 1. 取100ml TBSTx (10X),倒入到一洁净的量筒中,加入蒸馏水或去离子水混匀并定容到1升后即为TBSTx (1X)。没有用完的TBSTx (1X)可以室温保存,通常一周内是可以正常使用的,4℃可以保存更长时间。如果发现洗涤液出现浑浊、沉淀等异常现象,须废弃。
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TBSTx(TBS with Triton X-100) 100ml

TBSTx(TBS with Triton X-100) 100ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 100ml.

item number:T743333-100ml
Product model:100ml
level: 100ml
Lead Time:30days
sold 687 Items
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Specifications:

100ml

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