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Product details

名称

T7 Endonuclease I

别名

T7核酸内切酶I

英文别名

T7 Endo I | T7EI | T7p19

货号

T750878-20×250U

包装规格

20×250U

级别

无动物源

浓度

10 U/µL

产品介绍

本公司生产的 T7 Endonuclease I,即 T7 核酸内切酶 I，是一种比较特殊的 DNA 内切酶，能识别并切割不完全配对的 DNA(non-perfectly matched DNA)、十字型结构DNA(cruciform DNA structures)、Holliday结构或交叉DNA(Holliday structures or junctions)和异源双链DNA(heteroduplex DNA)。T7EI 切割的位点位于错配碱基 5’端的第一、第二或第三个磷酸二脂键。此外，T7EI 也能以很慢的速度切割含有缺刻的双链 DNA(nicked double-stranded DNA)。本产品常用于 CRISPR/Cas9 等导致的基因突变的鉴定。需要特别注意的是 T7EI 能识别长度大于或等于 2bp 的插入、缺失或突变导致的错配 DNA，不能识别1bp 的插入、缺失或突变。纯度（Purity）：不含其它 DNA 内切酶与外切酶活性，不含 RNA 酶活性。组分和说明 T750878

生物活性

One unit is defined as the amount of enzyme required to convert > 90% of 1μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37℃.

来源

重组表达

表达系统

E. coli Accession #: P00641.1 | E. coli

种属

Escherichia phage T7

无动物源

Yes

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

user reviews

Consultation

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After Sales Services

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Service Hotline:400-6226-992 ，We will handle it for you as soon as possible, thank you for your support and trust.！