嗜热菌蛋白酶 from Geobacillus stearothermophilus

T755345-25mg

25mg

1%/280)">Type X, lyophilized powder, 30-350 units/mg protein (E1%/280)

非特异性蛋白酶活性测定视频一种含有四个钙离子的热稳定性（嗜热性）细胞外金属内肽酶。辅助因子是锌和钙。水解疏水性氨基酸残基N-末端的蛋白质键。最适pH为8.0，活性的最适温度为70℃。在pH 5-9.5范围内非常稳定。嗜热菌蛋白酶具有较低的切割特异性，因此，它能产生许多适合测序的短片段。优先切割顺序： X-切割-Y-Z，其中X = 任何氨基酸；Y = Leu，Phe，Ile，Val，Met，Ala；Z是除Pro以外的任何氨基酸。切割Leu的N末端优先于切割Phe的N末端，它们优于其他氨基酸。常用于限制性蛋白水解以绘制肽图谱以及蛋白质结构和构象变化研究。嗜热菌蛋白酶也常用于商业合成N-（苄氧基羰基）-L-天冬氨酰-L-苯丙氨酸甲酯——人造甜味剂阿斯巴甜的前体。 已有研究显示嗜热菌蛋白酶具有前序列，可在体内充当分子内分子伴侣。 它还被用于研究氯化钠对其热稳定性和催化活性的影响。应用： 嗜热菌蛋白酶是一种蛋白酶，其特异性不同于可用于序列研究的其他蛋白酶。 Thermolysin is a protease that has specificity different from other proteases available for sequence investigations. Application: Non-specific Protease Activity Assay Video A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes. Thermolysin has been shown to have a prosequeence that acts as an intramolecular chaperone in vivo . It has also been used in a study to investigate the effects of sodium chloride on thermal stability and catalytic activity. Thermolysin is also commonly used for the commercial synthesis of N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester, the precursor for the artificial sweetener aspartame.