3,3′,5,5′-四甲基联苯胺 二盐酸盐

T755451-100tabs

100tabs

tablet, 1 mg substrate per tablet

3,3′,5,5′-四甲基联苯胺二盐酸已作为底物用于小鼠血清ELISA检测中的小鼠IgG（γ特异性）辣根过氧化物酶偶联抗体初始和效应T细胞裂解物的蛋白酪氨酸磷酸酶检测中的小鼠过氧化物酶抗体糖尿病患者血清样本的氢过氧化物酶法(EH)检测和氧化/抗氧化平衡(PAB)检测3,3′,5,5′-四甲基联苯胺 (TMB) 是一种适用于酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应（变成黄色），并在 450nm 处读取数据。 3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients 3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H 2 SO 4 (resulting in a yellow color), and read at 450 nm.