ELISA 用 3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物系统

T755522-100ml

100ml

peroxidase substrate

TMB底物可用于利用辣根过氧化物酶结合物进行的ELISA程序。TMB底物会形成可溶的蓝色反应产物，可通过370或655 nm处的吸光度测定。该试剂可与样品中的亚硝酸盐反应，形成紫色的偶氮产物，可在450 nm处测量其吸光度。该反应可利用酸终止，形成黄色反应产物，并可通过450 nm处的光强精确测定。我们的TMB底物使用方法：我们的TMB底物以即用型过氧化物酶底物的形式提供，其中的TMB溶于弱酸性缓冲液中。与过氧化物酶反应之前，底物溶液应呈无色至浅蓝绿色。与过氧化物酶反应时，底物系统形成蓝色反应产物。加入酸终止反应后，测量TMB底物在450 nm处的光强。我们的TMB底物推荐用于ELISA（微孔）程序，且已应用在各种测定方案中，如作为辣根过氧化物酶抗体偶联物的底物，用于检测亨德拉病毒糖蛋白、十四糖、sIgA和嗜中性白细胞胞外阱(NETs)。 不建议采用3,3′,5,5′-四甲基联苯胺(TMB)液体底物系统用于膜应用。由于TMB底物会产生可溶性反应产物，因此不建议用于组织化学或印迹分析。注意：TMB底物为光敏材料，应避免接触直射阳光或紫外线。应用： TMB底物是辣根过氧化物酶-HRP的显色底物。 TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP. Application: TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm. How to use our TMB substrate: Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase. Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm. Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs). 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System is not recommended for membrane applications. Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting. Note :TMB substrate is light-sensitive and should be protected from direct sunlight or UV sources.