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Product details

名称

泛素激活酶UBE1（人重组）

货号

U388907-50μl

包装规格

50μL

级别

EnzymoPure™

浓度

0.5 μg/μl in PBS containing 0.01% sodium azide

产品介绍

UBE1负责泛素-蛋白质异肽键形成的第一步。UBE1通过形成高能硫酯键来催化遍在蛋白C端羧基的活化。然后，该活化的遍在蛋白通过E2 / E3偶联级联转移到靶蛋白的赖氨酸上。引发体外反应的关键成分。 This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

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