亚叶酸钙盐水合物已被用于制备外用的1％和2.5％亚叶酸乳膏，可用于伤口愈合研究。它还被用作化学成分明确的中国仓鼠卵巢培养基（CD-CHO）的成分，用于培养CHO DUKX B11。叶酸拮抗剂通过结合二氢叶酸还原酶并阻滞叶酸转化为四氢叶酸盐发挥作用，而亚叶酸钙则可抵消叶酸拮抗剂的作用。在医学上，亚叶酸钙在甲氨蝶呤治疗后用于降低甲氨蝶呤的毒性。亚叶酸钙还可用于增强癌症治疗中 5-氟尿嘧啶的细胞毒性效应。应用： 5-甲酰基-5,6,7,8-四氢叶酸的非对映异构体的（6R，S）-混合物钙盐。亚叶酸，也称亚叶酸钙，通常以无定形粉末形式存在并且没有气味。这种维生素B9的活化形式为灰白色至浅米色。叶酸（FA）是水溶性维生素。亚叶酸是四氢叶酸的5-甲酰基衍生物，是叶酸的还原形式。 Calcium salt of the (6R,S)-mixture of diastereomers of 5-formyl-5,6,7,8-tetrahydrofolate. Folinic acid, also called as calcium leucovorin, is usually available as an amorphous powder and lacks odor. This activated form of vitamin B 9 is off-white to light beige in color. Folic acid (FA) is a water-soluble vitamin. Folinic acid is the 5-formyl derivative of tetrahydrofolic acid and is the reduced form of folic acid. Application: Folinic acid calcium salt hydrate has been used in the preparation of topical 1% and 2.5% folinic acid creams for use in wound healing studies. It has also been used as a component in the chemically defined-Chinese hamster ovary medium (CD-CHO) to culture CHO DUKX B11. Calcium folinate offsets the effects of folic acid antagonists that act by binding dihydrofolate reductase and blocking the conversion of folic acid to tetrahydrofolate. In medicine, calcium folinate is used after treatment with methotrexate to reduce the toxicity of the methotrexate. Calcium folinate is also used to enhance the cytotoxic effect of 5-fluorouracil in the treatment of cancer.