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Product details
名称
WB改良型促凝剂
别名
Western blot 改良型促凝剂 | 过硫酸铵替代物
英文别名
Ammonium persulfate substitute | APS substitute
英文名称
WB Modified Accelerator
货号
W1371750-100ml
包装规格
100ml
级别
适用于电泳
储存温度
2-8°C储存
运输条件
冰袋运输
产品介绍
我们生产的 Western blot 改良型促凝剂,是专门为PAGE-快速凝胶试剂盒量身订制,取代传统的过硫酸铵(AP),和传统过硫酸铵(AP)相比稳定性更好,4℃保存,使用更方便。 产品特色: 1.使用方便——即用型液体,无需配置。 2.冷藏保存——4℃保存,随取随用,方便快捷,不需要和试剂盒分开保存,避免出现制胶时找不到促凝剂的尴尬。 3.效果稳定----4℃稳定保存一年。 操作步骤: 1.下层胶配制:(以配置-块mini胶为例0.75/1.0/1.5 mm) a.等体积吸取下层胶溶液和下层胶缓冲液,混匀,即各吸取2/2.7/4mL。 b.对应加入40/60/80uL的促凝剂,混匀。 c.将配置好的溶液,注入制胶玻璃板中,(备注:此溶液配置为过量,留少许于小量杯中,以判断凝胶状态),加入适量水或醇覆盖于下层胶之上,待下层胶凝固后倒去上层水或醇,用滤纸吸去多余的水或醇(备注:当水或醇和胶之间有一条折射线时说明胶已凝固,大约15min左右,以具体实际情况为准)。 2.上层胶的配置: a.等体积吸取上层胶溶液和上层胶缓冲液混匀,即各吸取0.5/0.75/1 mL b.对应加入10/15/20uL的促凝剂,混匀。 c.将配置好的溶液,注入制胶玻璃板中,插入梳子,待上层胶凝固后拔取梳子(大约15min左右,以具体实际情况为准),便可进行上样电泳。 凝胶速度与温度有显著的正相关性。相同条件下,温度越高,凝胶速度越快,室温过高时建议适当减小改良型促凝剂的用量;相反,如果室温较低,可适当延长凝胶时间。 注意事项: 1.-20℃保存,有效期则更长。 2.为了您的安全和健康,请穿实验服并戴一次性手套操作。
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Research chemical 100ml

Research chemical 100ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 100ml.

item number:W1371750-100ml
Product model:100ml
level: 100ml
Lead Time:30days
sold 8 Items
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100ml

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