Wortmannin是一种PI3K抑制剂，IC50为3 nM，抑制自噬体的形成，也有效抑制DNA-PK/ATM，IC50分别为16 nM和150 nM。 一种细胞渗透性真菌代谢物，在纯化制剂和细胞溶质部分（IC50=5 nM）中，作为磷脂酰肌醇3-激酶的有效、选择性和不可逆抑制剂。阻断PI 3激酶的催化活性，而不影响上游信号事件。成纤维细胞与渥曼青霉素预孵育可消除PDGF介导的Ins（3,4,5）P3形成（IC50=5 nM）。在不影响胰岛素受体酪氨酸激酶活性的情况下，也可阻断胰岛素在离体大鼠脂肪细胞中的代谢作用。抑制血小板激活因子诱导的豚鼠中性粒细胞MAP激酶活化活（200-300nm）。也抑制其他激酶，如肌球蛋白轻链激酶（IC50=200 nM）和PI 4激酶，其浓度高于抑制PI 3激酶所需的浓度。还可阻断磷脂酶D。 Wortmannin is the first described PI3K inhibitor with IC50 of 3 nM, with little selectivity within the PI3K family. Also blocks autophagosome formation and potently inhibits DNA-PK/ATM with IC50 of 16 nM and 150 nM. A fungal metabolite which inhibits Pl3-kinase A cell-permeable fungal metabolite that acts as a potent, selective and irreversible inhibitor of phosphatidylinositol 3-kinase in purified preparations and cytosolic fractions (IC50=5 nM). Blocks the catalytic activity of PI 3-kinase without affecting upstream signaling events. Preincubation of fibroblasts with wortmannin abolishes PDGF-mediated Ins(3,4,5)P3 formation (IC50=5 nM). Blocks the metabolic effects of insulin in isolated rat adipocytes without affecting insulin receptor tyrosine kinase activity. Inhibits platelet-activating factor-induced MAP kinase activation in guinea pig neutrophils (200-300 nM). Also inhibits other kinases such as myosin light chain kinase (IC50=200 nM) and PI 4-kinase at concentrations higher than those required for inhibition of PI 3-kinase. Also blocks phospholipase D.