蛋白定量试剂盒

试剂盒

Wonderorangetm Protein Quantification Kit

W598072-1000T

1000T

避光,室温

常规运输

对光敏感

WonderOrange Protein Quantitation Kit 是一个高度敏感的基于荧光技术定量纯化蛋白质的试剂盒，其检测蛋白浓度范围为 0.1-10μg/mL。比起传统定量方法如 BCA、Bradford或 Lowry 蛋白定量分析，WonderOrange 蛋白定量试剂盒敏感性更佳，此外，与 NanoOrange 蛋白质定量测定技术相比具有更加优秀的线性和重现性。 WonderOrange 蛋白定量试剂盒可显示不同蛋白质之间的最小变异性，且荧光信号稳定长达 16 h；其受测样本不论为纯化蛋白或抗体皆适用。 组分： 产品参数： Ex/Em 480/598 nm（结合 BSA），图 1是WonderOrange 与 BSA 在 1×WonderOrange assay buffer 条件下的光谱图。 使用方法： 1. 准备 1×WonderOrange 缓冲液：1:10 稀释10×缓冲液在dH2O 中。例如，添加 1 mL 10×缓冲液至9 mL dH2O。注：A 组分 WonderOrange buffer, 10×容易产生沉淀，使用时可 50℃加热，沉淀解后使用。 2. 使用前准备 WonderOrange 工作液：将 200×WonderOrange 染料按 1:200 用 1×WonderOrange 缓冲液稀释。例如，添加25 μL 200×WonderOrange 染料至 5 mL 1×WonderOrange 缓冲液。 注意：您将需要大约 3 mL 工作液制作标准曲线（见表 1）以及每孔检测样品 250 μL 工作液。 3. 准备未知样本：10 μL 样本添加 250 μL WonderOrange 工作液。 注意：您可能需要稀释未知样本以得到不同浓度的样本。样品稀释可能会减少干扰物质的影响。 4. 准备测蛋白标准曲线所需的BSA 浓度，如表1 所示。 5. 样本和标准蛋白加热至 90℃- 95℃10 min，此过程需避光操作。 6. 取出样本室温避光放置冷却。短时离心收集全部样本。7. 每个标准样品或未知样品取200 μL 转移到96 孔酶标板，荧光酶标仪读数。激发/发射波长为480/598 nm。 注意：另外，样品可以被转移到荧光试管使用荧光仪测量。如果需要超过 200 μL 体积测量，等比例放大配制方案。 应用范围： 蛋白定量试剂盒、蛋白检测 Wonderorange Protein quantification kit is a highly sensitive kit based on fluorescence technology for quantitative purification of proteins, and its detection protein concentration range is 0.1-10 μ G/ml. Compared with traditional quantitative methods such as BCA, Bradford or Lowry protein quantitative analysis, the wonderorange protein quantitative kit is more sensitive. In addition, it has better linearity and reproducibility compared with nanoorange protein quantitative determination technology. The wonderorange protein quantification kit can show the minimum variability between different proteins, and the fluorescence signal is stable for up to 16 h; The tested sample is applicable to both purified protein and antibody. It should be noted that wonderorange has different degrees of tolerance to salts, buffers, detergents or other chemicals. Component： Product parameters： Scope of application： Ex/em 480/598 nm (bound to BSA)，Figure 1 The spectra of WonderOrange and BSA under the condition of 1 × WonderOrange assay buffer. Instruction： 1.Preparation of 1 × WonderOrange buffer : 1 : 10 diluted 10 × buffer in dH2O. For example, add 1 mL 10 × buffer to 9 mL dH2O. Note : A component WonderOrange buffer, 10 × is easy to produce precipitation. It can be heated at 50 °C and used after precipitation. 2.Preparation of WonderOrange working solution before use : Dilute 200 × WonderOrange dye at 1 : 200 with 1 × WonderOrange buffer. For example, add 25 μL 200 × WonderOrange dye to 5 mL 1 × WonderOrange buffer. Note : You will need approximately 3 mL of working fluid to make the standard curve ( Table 1 ) and 250 μL of working fluid per well of the test sample. 3.Preparation of unknown samples : 250 μL WonderOrange working solution was added to 10 μL samples. Note : You may need to dilute unknown samples to obtain samples of different concentrations. Sample dilution may reduce the impact of interfering substances. 4.Prepare the BSA concentration required for the determination of the protein standard curve, as shown in Table 1. 5.The sample and standard protein were heated to 90 °C -95 °C for 10 min, and this process needed to be operated in dark. 6.Take out the sample and place it in the dark at room temperature to cool. All samples were collected by short-time centrifugation. 7.200 μL of each standard sample or unknown sample was transferred to a 96-well microtiter plate and read by a fluorescence microplate reader. The excitation / emission wavelengths are 480 / 598 nm. Note : In addition, the sample can be transferred to a fluorescence test tube using a fluorescence meter for measurement. If more than 200 μL volume measurement is required, the proportional amplification configuration scheme. Scope of application： Protein Quantification Kit, protein detection