UltraBio™ Western封闭液

W751598-100ml

100ml

阿拉丁生产的UltraBio™ Western封闭液(UltraBio™ Blocking Buffer for Western Blot)是最新一代的快速高效Western封闭液，总体效果显著优于传统的基于BSA(牛血清白蛋白)、脱脂奶粉、酪蛋白(Casein)等的封闭液及国外同类产品，主要用于Western blot (WB)实验中PVDF膜或硝酸纤维素膜(nitrocellulose membrane, NC膜)的封闭，也可以用于Western实验中一抗或二抗的稀释。UltraBio™ Western封闭液快速高效。封闭时间通常仅需5-15分钟，并且和BSA、脱脂奶粉、酪蛋白等传统的Western封闭液以及国外同类的快速封闭液相比，显示出更强的信噪比(参考图1)。UltraBio™ Western封闭液封闭后背景极低。本封闭液不含血清和白蛋白，确保极高的信噪比。UltraBio™ Western封闭液兼容性好，兼容辣根过氧化物酶(Horseradish peroxidase, HRP)、碱性磷酸酶和生物素标记的二抗。本产品中添加了不影响HRP和AP活性的防腐剂，不会干扰HRP或AP标记二抗的检测。同时本产品不含生物素，不会干扰基于生物素的检测。UltraBio™ Western封闭液使用便捷。本产品无需添加任何额外的试剂，可以直接用于印迹膜的封闭。本产品与BSA及国外同类产品的封闭效果对比参见图1。在相同样品和实验条件下，仅封闭液及封闭时间存在如下图所示的差异时，阿拉丁的UltraBio™ Western封闭液封闭后的整体背景明显低于BSA封闭后的背景，而且目的条带亮度明显高于国外同类品牌产品。 图1. UltraBio™ Western封闭液与BSA及国外同类产品的封闭效果比较。每组实验从左到右依次为：5μl蛋白Marker，2.5μg蛋白量的HeLa细胞裂解液，5μg蛋白量的HeLa细胞裂解液。请注意对于Antibody A，UltraBio™封闭液比BSA和国外同类产品具有更低的背景，并且和国外同类产品相比信号明显更强。对于Antibody B，UltraBio™封闭液比BSA具有更低的背景，和国外同类产品相比信号明显更强。实际实验结果会因样品、抗体、实验条件等的不同而存在差异，图中数据仅供参考。关于不同封闭液的比较和选择，请参考阿拉丁的相关网页：http://www.aladdin-e.com/。按照每张膜封闭需要5-10ml UltraBio™ Western封闭液计算，一个100ml包装的本产品可以封闭10-20张膜，一个500ml包装的本产品可以封闭50-100张膜。 注意事项 ： 通常本产品用于PVDF膜及NC膜时的封闭时间为5-15分钟。对于一些背景非常高的抗体，可以尝试将封闭时间延长为30-60分钟。此外，如有特殊需要，也完全可以4℃封闭过夜。由于没有任何一种封闭液是适用于所有实验体系的，因此对于一些特殊的实验，可能需要根据具体情况考虑使用其它更合适的封闭液。取放PVDF膜和NC膜应使用平头镊子，并仅轻轻夹取其边角，操作过程须避免膜表面产生划痕、折痕或压痕等痕迹。PVDF膜一经浸润和活化，需一直保持湿润，根据Western进行到的具体步骤可放置于western转膜液或洗涤液等适当溶液中，否则可能会产生难以封闭的异常背景。为进一步提高信噪比，推荐同时使用QuickBlock?Western一抗稀释液和QuickBlock? Western二抗稀释液进行一抗及二抗的稀释。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明 ： 1.完成转膜后，用Western洗涤液洗涤印迹膜1-2分钟。2.根据膜的大小，在平皿或者其它适当容器中倒入一定体积的QuickBlock™ Western封闭液，确保封闭液后续能充分覆盖膜即可。对于常规的western，一张约6.6×8.5cm的膜推荐使用约10ml左右的封闭液。3.用平头镊夹住膜的一角，将膜放置在QuickBlock™ Western封闭液中，使封闭液完全浸没膜，置于水平摇床上封闭约10分钟(通常5-15分钟均可；经多种抗体的测试封闭10分钟的效果很多时候会显著优于常规的BSA封闭1小时的效果)。4.封闭后的膜即可用于一抗孵育等后续实验。详细的Western操作可以参考如下的相关网页：http://www.aladdin-e.com/support/western.htm。 Aladdin's UltraBio™ Blocking Buffer for Western Blot is the latest generation of fast and efficient blocking solution, and its overall performance is significantly better than traditional blocking buffers based on BSA (bovine serum albumin), skimmed milk powder, or casein etc. It is mainly used for blocking of PVDF or NC membranes and dilution of antibodies in Western blot (WB).UltraBio™ Blocking Buffer for Western Blot is fast and efficient. The blocking procedure takes only 5-15 minutes. This product delivers higher signal-to-noise ratio than traditional blocking solutions based on BSA, skimmed milk powder or casein, and other fast blocking systems (Figure 1).Background noise is very low after blocking with UltraBio™ Blocking Buffer for Western Blot. This blocking solution does not contain serum and albumin, ensuring a very high signal-to-noise ratio.UltraBio™ Blocking Buffer for Western Blot is compatible with secondary antibodies labeled with horseradish peroxidase (HRP), alkaline phosphatase (AP) or biotin. Preservatives contained in this product does not interfere the activity assay of HRP or AP conjugated with secondary antibodies. This Blocking Buffer is biotin free, which makes it compatible with avidin/streptavidin systemsUltraBio™ Blocking Buffer for Western Blot is flexible to use. This product is a ready-to-use liquid and can be directly used for blocking Western Blot membranes.Western Blot results obtained using three different blocking solutions are shown in Figure 1. Except for blocking solutions and the duration of blocking, all other experimental conditions are the same. As demonstrated in Figure 1, Aladdin's UltraBio™ Blocking Buffer delivers a higher signal-to-noise ratio than BSA and Competitor T’s blocking solution.Figure 1. Western Blot results obtained using three different blocking solutions Precautions ： Usually, it takes 5-15 minutes to block PVDF or NC membranes. For some antibodies with very high backgrounds, prolong the blocking time to 30-60 minutes, or overnight at 4ºC if necessary.Because no blocking reagent is optimal for all experimental systems, a proper blocking buffer should be selected based on the special requirement of your experiment. provides a variety of blocking solutions that you may compare and select.Use flat-tip tweezers to gently handle PVDF and NC membranes on the corners or edges to avoid producing any scrapes, creases or indentations on the surface of membrane.PVDF membrane, once infiltrated and activated, should be kept wet by placing the membrane in Western transfer buffer or washing buffer according to the Western Blot procedures. Otherwise, an abnormal background that is hard to be blocked may be produced.To improve the signal-to-noise ratio, we recommend using ’s QuickBlock™ Primary Antibody Dilution Buffer and QuickBlock™ Secondary Antibody Dilution Buffer to dilute primary and secondary antibodies, respectively.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use ： 1. After completing the protein transfer, wash the membrane with Western washing buffer for 1-2 minutes.2. Based on the size of the membrane, add a sufficient volume of QuickBlock™ Blocking Buffer for Western Blot in a petri dish or other appropriate container. For regular Western Blot, use 10ml of Blocking Buffer for a membrane of 6.6×8.5cm. 3. Clamp one corner of the membrane with a flat-tip tweezer and place the membrane in QuickBlock™ Blocking Buffer for Western Blot. Make sure that the membrane is covered completely by the Blocking Buffer. Incubate for 10 minutes with agitation on a horizontal shaker. The incubation time can be from 5 to 15 minutes. Generally, 10 minutes of blocking can achieve better blocking results than 1 hour’s blocking with BSA.4. The blocked membrane can be used for subsequent procedures such as primary antibody incubation. For detailed Western Blot procedures, please visit our website at http://www.aladdin-e.com