Xylopine is an aporphine alkaloid with cytotoxic activity on cancer cells. Xylopine induces oxidative stress, causes G2/M cell cycle arrest and apoptosis in cancer cells. In Vitro Xylopine (3.5 μM-14 μM; 24-48 hours) displays potent cytotoxicity in a time- and does-depenpent manner. Xylopine (72 h) has cytotoxic activity, with IC50 values ranging from 6.4 to 26.6 μM in eight different cancer cell lines (MCF7, HCT116, HepG2, SCC-9, HSC-3, HL-60, K-562, and B16-F10). Xylopine (3.5 μM-14 μM; 24-48 hours) causes cell cycle block at the phase G2/M, which is followed by internucleosomal DNA fragmentation. Xylopine (3.5 μM-14 μM; 24-48 hours) significantly increases the early and late apoptosis, induces mitochondrial depolarization, and increases caspase-3 activation. Xylopine also causes an increase in the production of reactive oxygen/nitrogen species (ROS/RNS), including hydrogen peroxide and nitric oxide, but not superoxide anion, and reduces glutathione levels are decreased in Xylopine-treated HCT116 cells.\nHCT116 cells3.5 μM, 7 μM, and 14 μM\n24 hours, 48 hours\nInduced G2/M phase arrest.\nHCT116 cells3.5 μM, 7 μM, and 14 μM\n24 hours, 48 hours\nSignificantly increased the early and late apoptosis. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HCT116 cells Concentration: 3.5 μM, 7 μM, and 14 μM Incubation Time: 24 hours, 48 hours Result: Displayed potent cytotoxicity in HCT116 cells. Cell Cycle AnalysisCell Line: HCT116 cells Concentration: 3.5 μM, 7 μM, and 14 μM Incubation Time: 24 hours, 48 hours Result: Induced G2/M phase arrest. Apoptosis AnalysisCell Line: HCT116 cells Concentration: 3.5 μM, 7 μM, and 14 μM Incubation Time: 24 hours, 48 hours Result: Significantly increased the early and late apoptosis. Form:Solid