神经酰胺的合成主要由涉及棕榈酰辅酶A和L-丝氨酸的从头途径驱动，也由通过复杂鞘脂的再循环的挽救途径驱动。神经酰胺转运蛋白Cert介导C14从平滑内质网向高尔基体的转移。神经酰胺合酶CerS6酶参与C14神经酰胺的合成。 Ceramide synthesis is majorly driven by de novo pathway involving the palmitoyl-CoA and L-serine, and also by salvage pathway via recycling of complex sphingolipids. The ceramide transporter, Cert mediates the transfer of C14 from smooth endoplasmic reticulum to Golgi. Ceramide synthase CerS6 enzyme is involved in the synthesis of C14 ceramide C14 Ceramide (d18:1/14:0) is suitable for use • as a standard in high-performance liquid chromatography/tandem mass spectrometry (LC/MS2) for ceramide quantification from muscel biopsies • in lipid monolayer preparation for biophysical studies • in the morphological and dimensional evaluation studies