YO565 SE（YO565琥珀酰亚胺酯）

YO565SE (YO565 succinimidyl ester)

Y598205-1mg

1mg

避光,-20°C储存

超低温冰袋运输

提供多种性价比高的氨基反应染料，用于标记蛋白质和其他含胺化合物。YO Dye SE可以与氨基基团反应生成稳定的酰胺键。 注意事项： 1. 荧光染料激发与发射波长会受到溶剂的影响，使用不同溶剂，则激发与发射波长可能会发生变化。 2. 标记的蛋白如需储存，可以添加终浓度为2 mM NaN3作为防腐剂，放置于4℃避光保存。如需长期储存，请将溶液分成小份并在 -20℃ 下冷冻，避免反复冻融，尽可能保护染料偶联物免受光照。 3. 操作过程注意避光，避免产生气泡。 4. 层析柱装柱时，尽量使柱体均匀，柱面平整，无气泡、裂隙。 5. 上样时注意，当柱顶缓冲液与凝胶平面相切时再加样品，洗脱时，当样品走至与凝胶平面相切时再加洗脱液。 6. 影响标记效率的其他因素还包括：温度、反应时间、pH、荧光染料与蛋白的量等，需注意控制。 7. 为了您的安全和健康，请穿实验服并戴一次性手套。 应用范围： 蛋白核酸标记染料 Product introduction： Uelandy provides a variety of cost-effective amino reactive dyes for labeling proteins and other amine containing compounds. YO dyeSE can react with amino groups to form stable amide bonds. Matters needing attention： 1. the excitation and emission wavelengths of fluorescent dyes will be affected by solvents. If different solvents are used, the excitation and emission wavelengths may change. 2. if the labeled protein needs to be stored, NaN3 with a final concentration of 2 mm can be added as a preservative and stored at 4 ℃ away from light. For long-term storage, please divide the solution into small portions and freeze at -20 ℃, avoid repeated freezing and thawing, and protect the dye conjugate from light as much as possible. 3. avoid light and bubbles during operation. 4. when installing the chromatographic column, try to make the column body uniform, the column surface flat, and free of bubbles and cracks. 5. pay attention to adding the sample when the column top buffer is tangent to the gel plane. When eluting, add the eluent when the sample is tangent to the gel plane. 6. other factors affecting the labeling efficiency also include temperature, reaction time, pH, the amount of fluorescent dye and protein, etc., which should be controlled. 7. for your safety and health, please wear laboratory clothes and disposable gloves. Scope of application： Protein nucleic acid labeling dye