复溶：Reconstitution in 30% ACN/H2O . Z-DEVD-AFC是一种人工合成的多肽，其核心部分由四个氨基酸组成：Asp-Glu-Val-Asp（DEVD），连接至一个荧光团AFC（7-氨基-4-三氟甲基香豆素）。在细胞凋亡过程中，特别是一些半胱天冬酶（Caspases）激活的情况下，Z-DEVD-AFC会被切割释放出AFC，后者发出明亮的荧光，充当了凋亡进程的实时监测器。 Z-DEVD-AFC 是细胞渗透的 caspase-3 的底物，它会导致 AFC 荧光团的荧光向上移动。Z-DEVD-AFC 可用于检测 caspase-3 样酶的活性。 Z-DEVD-AFC is a synthetic polypeptide whose core part consists of four amino acids: Asp-Glu-Val-Asp (DEVD), linked to a fluorophore AFC (7-amino-4-trifluoromethylcoumarin). During the process of cell apoptosis, especially when some caspases are activated, Z-DEVD-AFC will be cleaved to release AFC, which emits bright fluorescence and acts as a real-time monitor of the apoptotic process. Z-DEVD-AFC is a cell-permeant substrate for caspase-3, which causes a shift in fluorescence uponcleavage of the AFC fluorophore. Z-DEVD-AFC can be used to detect caspase-3-like enzymes activity.