免疫球蛋白E(IgE),普通人血浆

np001077-25μg

25μg

≥95%(SDS-PAGE)

IgE是血浆中含量最低的免疫球蛋白，其浓度低于正常血浆的0.6微克/毫升。在经历严重过敏反应和寄生虫感染的患者中发现IgE水平升高。在骨髓瘤的情况下，IgE是由单个浆细胞克隆产生的。然而，骨髓瘤IgE的结构是正常的，从骨髓瘤来源纯化的免疫球蛋白是研究免疫球蛋白行为的有用蛋白质。 由经美国食品药品监督管理局批准的测试显示对HBsAg、抗-HCV、抗-HBc无反应且对抗-HIV 1和2呈阴性的血浆制备。 产品引用： Janssen, Kris PF, et al. "Multiplexed protein detection using an affinity aptamer amplification assay." Analytical and bioanalytical chemistry 404, no. 6-7 (2012): 2073-2081. Schachermeyer, Samantha, et al. "Aptamer-Protein Binding Detected by Asymmetric Flow Field Flow Fractionation."Journal of Chromatography A (2013). Abe, Takaaki, et al. "Antibody against immunoglobulin E contained in blood components as causative factor for anaphylactic transfusion reactions." Transfusion (2014). Kashiwakura, Jun-ichi, et al. "Most highly cytokinergic IgEs have polyreactivity to autoantigens." Allergy, asthma & immunology research 4, no. 6 (2012): 332-340. Oh SS, et al. Synthetic aptamer-polymer hybrid constructs for programmed drug delivery into specific target cells. J Am Chem Soc. 2014 Oct 22;136(42):15010-5. doi: 10.1021/ja5079464. Epub 2014 Oct 7. Cao J, Wang H, Liu Y. Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for detection of IgE with gold nanoparticles amplification. Spectrochim Acta A Mol Biomol Spectrosc. 2015;151:274-9. doi: 10.1016/j.saa.2015.06.104. Epub 2015 Jun 29. Liu YM, et al. Aptamer-based detection and quantitative analysis of human immunoglobulin E in capillary electrophoresis with chemiluminescence detection. Electrophoresis. 2015 Oct;36(19):2413-8. doi: 10.1002/elps.201500158. Epub 2015 Jul 29. Poongavanam MV, Kisley L, Kourentzi K, Landes CF, Willson RC. Ensemble and single-molecule biophysical characterization of D17.4 DNA aptamer-IgE interactions. Biochim Biophys Acta. 2016 Jan;1864(1):154-64. doi: 10.1016/j.bbapap.2015.08.008. Epub 2015 Aug 22. Garman L, Smith K, Muns EE, Velte CA, et al. Unique Inflammatory Mediators and Specific IgE Levels Distinguish Local from Systemic Reactions after Anthrax Vaccine Adsorbed Vaccination. Clin Vaccine Immunol. 2016 Aug 5;23(8):664-71. doi: 10.1128/CVI.00092-16. Print 2016 Aug. Ref: Ishiaka, K.1985. Methods Enzymol. 116, 76. IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. Product Citations: Janssen, Kris PF, et al. "Multiplexed protein detection using an affinity aptamer amplification assay." Analytical and bioanalytical chemistry 404, no. 6-7 (2012): 2073-2081. Schachermeyer, Samantha, et al. "Aptamer-Protein Binding Detected by Asymmetric Flow Field Flow Fractionation."Journal of Chromatography A (2013). Abe, Takaaki, et al. "Antibody against immunoglobulin E contained in blood components as causative factor for anaphylactic transfusion reactions." Transfusion (2014). Kashiwakura, Jun-ichi, et al. "Most highly cytokinergic IgEs have polyreactivity to autoantigens." Allergy, asthma & immunology research 4, no. 6 (2012): 332-340. Oh SS, et al. Synthetic aptamer-polymer hybrid constructs for programmed drug delivery into specific target cells. J Am Chem Soc. 2014 Oct 22;136(42):15010-5. doi: 10.1021/ja5079464. Epub 2014 Oct 7. Cao J, Wang H, Liu Y. Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for detection of IgE with gold nanoparticles amplification. Spectrochim Acta A Mol Biomol Spectrosc. 2015;151:274-9. doi: 10.1016/j.saa.2015.06.104. Epub 2015 Jun 29. Liu YM, et al. Aptamer-based detection and quantitative analysis of human immunoglobulin E in capillary electrophoresis with chemiluminescence detection. Electrophoresis. 2015 Oct;36(19):2413-8. doi: 10.1002/elps.201500158. Epub 2015 Jul 29. Poongavanam MV, Kisley L, Kourentzi K, Landes CF, Willson RC. Ensemble and single-molecule biophysical characterization of D17.4 DNA aptamer-IgE interactions. Biochim Biophys Acta. 2016 Jan;1864(1):154-64. doi: 10.1016/j.bbapap.2015.08.008. Epub 2015 Aug 22. Garman L, Smith K, Muns EE, Velte CA, et al. Unique Inflammatory Mediators and Specific IgE Levels Distinguish Local from Systemic Reactions after Anthrax Vaccine Adsorbed Vaccination. Clin Vaccine Immunol. 2016 Aug 5;23(8):664-71. doi: 10.1128/CVI.00092-16. Print 2016 Aug. Ref: Ishiaka, K.1985. Methods Enzymol. 116, 76.

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