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名称
酸性蛋白酶 来源于黑曲霉
英文别名
Aspergillus acid protease | Protease | Acidic Protease | Proteinase | pepA | aspergillopepsin A | aspergillopepsin F | aspergillopeptidase A | awamorin | proctase B | Protease type XIII | proteinase B | trypsinogen kinase
货号
np226936-250g
包装规格
250g
级别
工业级
浓度
≥50 U/mg powder
产品介绍
真菌酸性蛋白酶是由黑曲霉( Aspergillus niger)优良菌种经发酵精制提炼而成,它能在低pH条件下,有效水解蛋白质,广泛应用于谷物原料酒精、白酒、皮革加工、饲料添加、单细胞蛋白饲料、食品酿造等行业。 原理 酸性蛋白酶是活性中心含有天门冬氨酸的蛋白酶,可切开广泛氨基酸所构成的肽键,优先切开两端是芳香族或疏水性氨基酸残基之间的肽键,尤其优先切开由芳香族氨基酸与其他氨基酸所构成的肽键,产物为小肽和氨基酸。 产品特性 1.温度范围:有效温度范围30-65℃,最适作用温度范围在55-65℃之间。 2.pH值范围:有效pH范围 2.0-6.0,最适pH值范围2.5-3.5之间。 3.金属离子对酶活性的影响:酸性蛋白酶可被Ca2+、Mg2+、Mn2+激活,可被Cu2+、Hg2+、Al3+抑制。不宜长时间存放在碳钢罐中。 产品标准:执行中华人民共和国国家标准GB/T 23527-2009。 应用方法 1.在谷物发酵生产酒精中,用量一般为0.05-0.15L/t原料(酶活力以10万单位计)。间歇发酵在进料1/3时加入,连续发酵工艺在相当于第一个发酵罐的罐体中加入。 2.在醋、酱油、黄酒、白酒等食品酿造中,酸性蛋白酶的使用对促进菌体更健壮生长、提高曲的糖化发酵能力有明显作用,可达到提高原料利用率、改善风味的作用。推荐加量0.15-0.2L(kg)/t原料(酶活力以10万单位计),在制曲或发酵开始时加入。 3.在羊毛减量细化中,酸性蛋白酶可以破坏羊毛纤维表面的鳞片,使羊毛不宜毡缩,有利于染色。作为小剂量试验的起始最适使用条件:0.6-2.0%(owf)(酶活力以10万单位计),pH3.0-3.5,温度40-50℃,浴比1:20-40,处理60-90分钟,减量率达1-4%。 4.在饲料复合酶中,该酶所占的添加量一般为0.0025-0.01kg/t全价料(酶活力以10万单位计)。 5.在单细胞蛋白饲料生产中,添加量0.1-0.2L(kg)/t原料(酶活力以10万单位计),同菌种泼洒到灭过菌已冷却的料醅中,拌匀后入池发酵。可增加蛋白含量4-10%。 应用注意 1.请严格按照安全使用说明书操作,避免吸入酶粉尘与酶的气溶胶,不慎溅到手上或眼中,应马上用水冲洗15分钟以上。 2.发酵行业使用酸性蛋白酶时,发酵速度加快,因此发酵过程升温快,必须严格控制温度,使其不超过微生物的最适发酵温度,否则会影响发酵效果。 储存 本品为生物活性物质,高温、强酸、强碱会引起酶失活。因此在运输和储存过程中,液体酶应避免日光曝晒,固体酶需避免暴晒和雨淋。仓库应保持清洁、阴凉和干燥。
生化机理
Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions, but also accepts a lysine residue in the P1 position, leading to the activation of trypsinogen and chymotrypsinogen A.
生物活性
≥50 U/mg powder
来源
天然
种属
黑曲霉(Aspergillus niger)
Safety
「No Dangerous Attributes」
Related
「No upstream and downstream information yet」
Technical Documents
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Articles
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Research chemical 250g

Research chemical 250g; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 250g.

item number:np226936-250g
Product model:250g
level: 250g
Lead Time:30days
sold 536 Items
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