Recombinant Human CCL28 Protein

重组人粘膜相关上皮趋化因子 | 重组人CCL28蛋白

CCL28 | chemokine (C-C motif) ligand 28 | MEC | member 28 | MGC71902 | Protein CCK1 | VIC | SCYA28 | C C motif chemokine ligand 28 | C-C motif chemokine 28 | CC chemokine CCL28 | CCK1 protein | CCL28_HUMAN | chemokine (C-C motif) ligand 28 splice variant

rp143745-50μg

50μg

无动物源

≥96%(SDS-PAGE&HPLC)

＞96% (SDS-PAGE, HPLC)

CCL28属于CC家族趋化因子，又称粘膜相关上皮趋化因子(MEC)。CCL28 cDNA位于人的chr5上，CCL28的mRNA在人和小鼠的正常和病理结肠， 唾液腺，乳腺，气管和直肠等不同组织的上皮细胞中高度表达。人和小鼠的CCL28氨基酸序列在蛋白质被加工成成熟形态时，具有83%的同源性。CCL28是通过CCR3和CCR10信号转导，以及静息CD4、CD8 t细胞和嗜酸性粒细胞的趋化。MEC/ CCL28含有6个半胱氨酸，包括4个高度保守的半胱氨酸残基CC趋化因子。重组人MEC/ CCL28蛋白为12.3kDa，含有108个氨基酸残基； Purity >96% SDS-PAGE. estimated also by HPLC.Endotoxin level is less than 0.1 ng per µg (1EU/µg). Function Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 cDNA is located on Chr.5 in humans, and the mRNA of CCL28 is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83 % identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils. MEC/ CCL28 contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines. Recombinant Human MEC/ CCL28 is a 12.3kDa protein containing 108 amino acid residues. .

Fully biologically active when compared to standard. Determined by its ability to chemoattract human lymphocytes using a concentration range of 1.0-10.0 ng/ml.

重组表达

12.3kDa

No tag

12.9 kDa, under reducing conditions; 22.2 kDa, under non-reducing conditions.

E. coli Accession #: Q9NRJ3 | E. coli

<1.0 EU/μg

人(Human)

20-127 aa

SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNHTVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY

Yes

Yes