Recombinant Human CHST15 Protein

碳水化合物磺基转移酶15重组蛋白

B cell RAG associated protein (GALNAC4S-6ST) | B-cell RAG-associated gene protein | BRAG | BRAGKIAA0598RP11-47G11.1 | carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 | Carbohydrate Sulfotransferase 15 | CHST15 | DKFZp781H1369 | EC 2

rp144361-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.

Measured by its ability to transfer sulfate from PAPS to chondroitin sulfate. The specific activity is >100 pmol/min/μg, as measured under the described conditions.

重组表达

55 kDa

N-His

61 - 82 kDa, under reducing conditions; 60 - 76 kDa, under non-reducing conditions.

HEK293 Accession #: Q7LFX5 | HEK293

<0.1 EU/μg

人(Human)

99-561 aa

HHHHHHSGAHQELLISSPFHYGGFPSNPSLMDSENPSDTKEHHHQSSVNNISYMKDYPSIKLIINSITTRIEFTTRQLPDLEDLKKQELHMFSVIPNKFLPNSKSPCWYEEFSGQNTTDPYLTNSYVLYSKRFRSTFDALRKAFWGHLAHAHGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVKFSAIKEPHWWTRKRFGIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASASTMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVERLYSDYLYFASSNKSADDFHEKVTEALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLSVFDKQQFLILRLEDHASNVKYTMHKVFQFLNLGPLSEKQEALMTKSPASNARRPEDRNLGPMWPITQKILRDFYRPFNARLAQVLADEAFAWKTT

Yes

Yes