Recombinant Human GM-CSF Protein

重组人粒巨噬细胞集落刺激因子 | 重组人 GM-CSF 蛋白

Human Granulocyte-Macrophage Colony Stimulating Factor | Colony stimulating factor 2 (granulocyte-macrophage) | Colony-stimulating factor | CSF | CSF2 | CSF-2 | GMCSF | GM-CSF | GMCSFgranulocyte-macrophage colony-stimulating factor | granulocyte-macrophag

rp146523-5μg

5μg

无动物源

≥98%(SDS-PAGE&HPLC)

＞98% (SDS-PAGE, HPLC )

人粒细胞-巨噬细胞集落刺激因子(GM-CSF)由多种不同类型的细胞(包括活化的T细胞、B细胞、巨噬细胞、肥大细胞、内皮细胞和成纤维细胞)在细胞因子或免疫和炎症刺激下分泌。它最初被认为是一种生长因子，可以支持粒细胞-巨噬细胞祖细胞的体外集落形成，并具有刺激各种造血前体细胞生长和分化的功能。GM-CSF也有报道在非造血细胞上有功能作用，可以诱导人内皮细胞迁移和增殖。此外，它可以刺激多种肿瘤细胞系的增殖，包括骨原性肉瘤、癌和腺癌细胞系。GM-CSF被用作一种药物，在化疗后刺激白细胞的产生，最近也在临床试验中评估了它作为hiv感染患者疫苗佐剂的潜力。重组人GM-CSF是非糖基化多肽链，包含127个氨基酸。 Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Human Granulocyte-Macrophage Colony Stimulating Factor Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy and has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The recombinant Human GM-CSF is a non-glycosylated polypeptide chain containing 127 amino acids.

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is typically < 0.2ng/mL.

重组表达

14.5 kD

No tag

13.7 kDa, under reducing conditions; 12.3 kDa, under non-reducing conditions.

Full length protein

E. coli Accession #: P04141 | E. coli

<0.01 EU/μg

人(Human)

18-144 aa

APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFD LQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSC ATQIITFESFKENLKDFLLVIPFDCWEPVQE

Yes

Yes