Recombinant Human PCNA Protein

重组人PCNA蛋白 | 重组人类PCNA蛋白

PCNA, Cyclin, Proliferating cell nuclear antigen | ATLD2 | cb16 | DNA polymerase delta auxiliary protein | etID36690.10 | fa28e03 | fb36g03 | HGCN8729 | MGC8367 | Mutagen-sensitive 209 protein | OTTHUMP00000030189 | OTTHUMP00000030190 | Pcna/cyclin | PCNA

rp149860-50μg

50μg

无动物源

≥95%(SDS-PAGE)

>95% (SDS-PAGE)

Purity : >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Function : This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Description: Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.

重组表达

32.3 kDa

N-His

35.9 kDa, reducing conditions；35.9 kDa，non-reducing condition

E. coli Accession #: P12004 | E. coli

人(Human)

1-261aa

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS

Yes

Yes