Recombinant Human IL-15 Protein

重组人IL-15蛋白 | 重组人白细胞介素15蛋白

IL15 | IL-15 | IL-15MGC9721 | interleukin 15 | interleukin-15

rp156000-10μg

10μg

无载体

≥85%(SDS-PAGE)

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly. IL-15 binds with high affinity to IL-15 R alpha. It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain (gamma c), which are also subunits of the IL-2 receptor complex. IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface. The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells. This enables cells to respond to IL-15 even if they do not express IL-15 R alpha. In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation. Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. An important component of these functions is the ability of IL‑15 to induce dendritic cell differentiation and inflammatory activation. IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells. It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity. Immunotherapy treatment with recombinant IL-15 has the advantage of not stimulating Treg cells like IL-2 does but has the drawback of associated toxicity at higher doses. This has led to increased investigation on mitigating IL-15 toxicity and combination immunotherapy approaches using immune checkpoint inhibitors. Preclinical and early clinical studies have shown the potential of also using IL-15 in combination with cancer vaccines to improve their anti-tumor response. IL-15 can also be used for the preconditioning of CAR T cells or for engineering cells to express IL-15 in vivo. Adoptive cell transfer of NK cells engineered to express CD19 and IL-15 were well tolerated in patients with CD19-positive cancers. IL-15 can be used in combination with other cytokines like IL-21 to increase the efficiency of NK cell expansion and maturation in stem cell culture protocols. The combination of IL-15 with IL-7 also promotes expansion of early-differentiated CD8+ T cells in culture with the added benefit of decreasing Treg cell generation, unlike IL-2, for adoptive cell transfer in cancer immunotherapy. GMP IL-7 and GMP IL-15 are commonly used in combination for ex vivo expansion of T cells for cellular therapies.

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 9.32 ng/mL.

重组表达

12.8 kDa

No tag

11.7 kDa, under reducing conditions; 11.7 kDa, under non-reducing conditions.

E. coli Accession #: P40933 | E. coli

<1.0 EU/μg

人(Human)

49-162 aa

NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS

No

Yes