Share to

Details

Safety

Technical Documents

Articles

reviews

Consult

returns

Product details

名称

Recombinant Human IL-1β Protein

别名

重组人IL-1β蛋白 | 重组人IL-1β蛋白

英文别名

货号

rp156012-10μg

包装规格

10μg

级别

无载体

浓度

≥95%(SDS-PAGE)

产品介绍

Protein Function: IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in humans. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

生物活性

1. Immobilized Recombinant Human IL-1β Protein (rp156012) at 5.0 μg/mL can bind Recombinant Human IL-1 RII Protein (rp181605) with the EC50 of 176.8 ng/mL. 2. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 52.68 ng/mL. 3. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Canakinumab (Anti-IL-1b) (Ab170512) with the EC50 of 22.81 ng/mL.

来源

重组表达

预测分子量

17.4 kDa

蛋白标签

No tag

SDS-PAGE

15.0 kDa, under reducing conditions; 15.1 kDa, under non-reducing conditions

表达系统

E. coli Accession #: P01584 | E. coli

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

117-269 aa

序列

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

无动物源

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

user reviews

Consultation

Warm reminder：

Because the manufacturer may temporarily adjust product specifications, prices, inventory, parameters and other information without notice, and there are differences in each user's consultation time and procurement needs, the platform reply content is only of reference value within a certain period. Sorry for the inconvenience caused to you, and thank you for your understanding and support! Please consult the online customer service for the latest details. Everything is subject to the real-time reply of the customer service.！

After Sales Services

Please check the quantity, appearance and product information in time when you receive the goods. In case of damage, wrong delivery, abnormal quality and other problems, please feel free to contact us.

Service Hotline:400-6226-992 ，We will handle it for you as soon as possible, thank you for your support and trust.！