Recombinant Human M-CSF Protein

重组人M-CSF蛋白 | 重组人巨噬细胞集落刺激因子1蛋白 | 重组人集落刺激因子1蛋白

colony stimulating factor 1 (macrophage) | CSF1 | CSF-1 | Lanimostim | macrophage colony stimulating factor | macrophage colony-stimulating factor 1 | MCSF | M-CSF | MCSFlanimostim | MGC31930

rp156168-10μg

10μg

无动物源

≥95%(SDS-PAGE)

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation, and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. It primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full-length human M-CSF transcripts encode a 522 amino acid type I transmembrane protein with a 464 amino acid extracellular region, a 21 amino acid transmembrane domain, and a 37 amino acid cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan to generate a 200 kDa subunit. Although proteoglycan-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lacks cleavage and proteoglycan sites and produces an N-glycosylated 68 kDa transmembrane dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 amino acid portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 amino acids of mature human M-CSF share 88%, 86%, 81%, and 74% amino acid identity with corresponding regions of dog, cow, mouse, and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific. Post-translational: N-glycosylated. O-glycosylated.

Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 21.77 ng/mL. Immobilized Recombinant Human M-CSF Protein (rp215098) at 1.0 μg/mL can bind Lacnotuzumab (anti-M-CSF) (Ab177849) with the EC50 of 37.0 ng/mL.

重组表达

26.0 kDa

C-His

33.4-50.6 kDa, under reducing conditions; 89.7-101.6 kDa, under non-reducing conditions.

HEK293 Accession #: P09603 | HEK293

<1.0 EU/μg

人(Human)

1-255 aa

MTAPGAAGRCPPTTWLGSLLLLVCLLASRSITEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCNCLYPKAIPSSDPASVSPHQPLAPSMAPVAGLTWEDSEGTEGSSLLPGEQPLHTVDPGSAKQRPPRHHHHHH

Yes

Yes