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Product details

名称

Recombinant Human IL-10 Protein

别名

重组人IL-10蛋白 | 重组人白细胞介素-10蛋白

英文别名

货号

rp156176-10μg

包装规格

10μg

级别

无动物源

浓度

≥90%(SDS-PAGE)

生化机理

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes IL‑19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155. IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Mature human IL‑10 shares 72% ‑ 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells. IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction. IL‑10 R beta also associates with IL‑20 R alpha, IL‑22 R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29. IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.

生物活性

Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind Recombinant Human IL-10 R alpha Protein (rp147368) with the EC50 of 323.7 ng/mL. Immobilized Recombinant Human IL-10 Protein (rp156176) at 5.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC50 of 30.3 ng/mL.

来源

重组表达

预测分子量

19.5 kDa

蛋白标签

C-His

SDS-PAGE

17.4 kDa, under reducing conditions; 17.7 & 16.3 kDa, under non-reducing conditions.

表达系统

HEK293 Accession #: P22301 | HEK293

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

19-178 aa

序列

SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRNHHHHHH

无动物源

Yes

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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